All lanes : Anti-CALCOCO2 antibody [GT422] (ab184688) at 1/1000 dilutionLane 1 : Jurkat whole cell lysateLane 2 : Raji whole cell lysateLane 3 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-CALCOCO2 antibody [GT422] (ab184688) at 1/1000 dilutionLane 1 : Huh7 whole cell lysateLane 2 : Hep3B whole cell lysateLane 3 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of 3 μg/ml Thapsigargin treated (right) or untreated (left) HepG2 cells (fixed in ice-cold MeOH for 10 min, permeabilized with cooled acetone for 1 min) labeling CALCOCO2 with ab184688 at 1/1000 dilution (Green). Blue: Hoechst 33342 staining.