Detection of IL-38/IL-1F10 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑38/IL‑1F10 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7774G, filled histogram) or isotype control antibody (Catalog # IC006G , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004 ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005 ). View our protocol for Staining Intracellular Molecules .