All lanes : Anti-Caldesmon antibody (ab133224) at 1/500 dilutionLane 1 : HeLa cell extracts, treated with EGF (200 ng/ml for 30 minutes)Lane 2 : HeLa cell extracts, treated with EGF (200 ng/ml for 30 minutes) with synthesized peptide
Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate, smooth muscle labelling Caldesmon with ab133224 at 10 ug/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescent analysis of HeLa cells labelling Caldesmon with ab133224 at 1/100 dilution. The image on the right is treated with the synthesized peptide.