Anti-Caldesmon antibody [E89]

• Supplier: Abcam
• Catalog: ab32330
• Prices: $394.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: E89
• Applications: WB IHC-P ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic phospho peptide corresponding to residues surrounding Ser789 of human Caldesmon
• Description: Rabbit Monoclonal
• Gene: CALD1
• Conjugate: Unconjugated

Product images

Anti-Caldesmon antibody [E89] (ab32330) at 1/20000 dilution + NIH3T3 cell lysate

ab32300, at a 1/250 dilution, staining human Caldesmon in uterus tissue by Immunohistochemistry, Paraffin embedded tissue

ab32330, at a 1/250 dilution, staining human Caldesmon in HeLa cells by Immunofluorescence

Overlay histogram showing HeLa cells stained with ab32330 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32330, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Fluorescent immunohistochemical analysis of paraffin-embedded human normal kidney vessels using ab32330. Green-Caldesmon red-PI

Fluorescent immunohistochemical analysis of paraffin-embedded human tissue using ab32330. Green-Caldesmon red-PI.

Fluorescent immunohistochemical analysis of paraffin-embedded human normal uterus tissue using ab32330. Green-Caldesmon red-PI.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

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