Anti-Calnexin antibody
| Name | Anti-Calnexin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58504 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide non-phosphopeptide derived from human Calnexin - ER membrane marker around the phosphorylation site of serine 583 (N-R-SP-P-R) |
| Description | Rabbit Polyclonal |
| Gene | CANX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Calnexin antibody (ab58504) at 1/500 dilutionLane 1 : HeLa cells treated with EDF (200ng/ml, 30mins), without immunising peptideLane 2 : HeLa cells treated with EDF (200ng/ml, 30mins), with immunising peptide
Ab58504 staining human normal placenta tissue. Staining is localised to intracellular compartment (endoplasmic reticulum).Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b
Product References
Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+ - Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+
Novgorodov SA, Chudakova DA, Wheeler BW, Bielawski J, Kindy MS, Obeid LM, Gudz TI. J Biol Chem. 2011 Feb 11;286(6):4644-58.
Novel pathway of ceramide production in mitochondria: thioesterase and neutral - Novel pathway of ceramide production in mitochondria: thioesterase and neutral
Novgorodov SA, Wu BX, Gudz TI, Bielawski J, Ovchinnikova TV, Hannun YA, Obeid LM. J Biol Chem. 2011 Jul 15;286(28):25352-62.
Mycobacterium tuberculosis synergizes with ATP to induce release of microvesicles - Mycobacterium tuberculosis synergizes with ATP to induce release of microvesicles
Ramachandra L, Qu Y, Wang Y, Lewis CJ, Cobb BA, Takatsu K, Boom WH, Dubyak GR, Harding CV. Infect Immun. 2010 Dec;78(12):5116-25.