CANX

Gene Symbol CANX
Entrez Gene 821
Alt Symbol CNX, IP90, P90
Species Human
Gene Type protein-coding
Description calnexin
Other Description major histocompatibility complex class I antigen-binding protein p88
Swissprots D6R9K3 B4DGP8 B4E2T8 P27824 D3DWQ3 B2R5V8
Accessions CCD35249 EAW53802 EAW53803 EAW53804 EAW53805 P27824 AF070646 AJ271880 CAB72137 AK129990 BAC85269 AK130080 AK294702 BAG57859 AK304420 BAG65250 AK312334 BAG35255 BC003552 AAH03552 BC042843 AAH42843 BC050534 DQ890627 ABM92244 DQ893794 ABM84720 L10284 AAA36125 L18887 AAA21013 M94859 AAA21749 M98452 AAA35696 S67659 AAB29309 XM_011534664 XP_011532966 XM_011534665 XP_011532967 NM_001024649 NP_001019820 NM_001746 NP_001737
Function Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. The palmitoylated form preferentially localizes to the perinuclear rough ER.
Top Pathways Phagosome, Protein processing in endoplasmic reticulum, HTLV-I infection, Thyroid hormone synthesis, Antigen processing and presentation

Calnexin Antibody - 2433 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Calnexin Antibody.

Calnexin (C5C9) Rabbit mAb - 2679 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C5C9
Western blot analysis of extracts from PANC1, HepG2 and A204 cells using Calnexin (C5C9) Rabbit mAb.

Calnexin (H-70) - sc-11397 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Calnexin (H-70): sc-11397. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.

Calnexin (C-20) - sc-6465 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calnexin (C-20): sc-6465. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.

Calnexin (AF18) - sc-23954 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calnexin (AF18): sc-23954. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.

Calnexin (3H4A7) - sc-130059 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calnexin (3H4A7): sc-130059. Western blot analysis of Calnexin expression in A-431 (A), HeLa (B), MCF7 (C) and A549 (D) whole cell lysates.

Calnexin (TO-5) - sc-80645 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calnexin (TO-5): sc-80645. Western blot analysis of Calnexin expression in HeLa (A), A-431 (B), K-562 (C) and Jurkat (D) whole cell lysates.

Calnexin (F-17) - sc-31081 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Calnexin (E-10) - sc-46669 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calnexin (E-10): sc-46669. Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Calnexin (6D195) - sc-70481 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calnexin (6D195): sc-70481. Western blot analysis of Calnexin expression in non-transfected 293T: sc-117752 (A), human Calnexin transfected 293T: sc-115588 (B) and HeLa (C) whole cell lysates.

Anti-Calnexin (phospho S583) antibody - ab58503 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Calnexin (phospho S583) antibody (ab58503) at 1/500 dilutionLane 1 : HeLa cell extract treated with EGF (200ng/ml, 30mins)Lane 2 : HeLa cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide

Anti-Calnexin antibody - ab58504 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Calnexin antibody (ab58504) at 1/500 dilutionLane 1 : HeLa cells treated with EDF (200ng/ml, 30mins), without immunising peptideLane 2 : HeLa cells treated with EDF (200ng/ml, 30mins), with immunising peptide

Anti-Calnexin antibody [6F12BE10] - ER Membrane Marker - ab112995 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6F12BE10
Immunoprecipitation with ab112995. Immunoprecipitation of Calnexin - ER membrane marker from HeLa cell lysate. The protein band runs around 90 kDa (predicted 68kDa) in tris-glycine SDS-PAGE. The identity of this protein was confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.

Anti-Calnexin antibody [AF18] - BSA and Azide free - ab177023 from Abcam

Prices $368.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone AF18

Anti-Calnexin antibody [AF18] - ER Membrane Marker - ab31290 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone AF18
All lanes : Anti-Calnexin antibody [AF18] - ER Membrane Marker (ab31290) at 5 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab52255)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Calnexin antibody [AF18] - ER Membrane Marker (Alexa Fluor® 647) - ab202572 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AF18
ab202572 staining Calnexin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202572 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-Calnexin antibody [EPR3632] - ab92573 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3632
All lanes : Anti-Calnexin antibody [EPR3632] (ab92573) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HepG2 cell lysates Lysates/proteins at 10 µg per lane.Secondarystandard HRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker - ab133615 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3633(2)
Overlay histogram showing HeLa cells fixed in 80% methanol and stained with purified ab133615 at a dilution of 1 in 360 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black line) and cells without incubation with antibody were used as a negative control (blue line).

Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (Alexa Fluor® 488) - ab184812 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3633(2)
Overlay histogram showing HeLa cells stained with ab184812 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184812, 1/5000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (HRP) - ab195198 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3633(2)
IHC image of Calnexin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195198 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Calnexin antibody [TO-5] - ab49748 from Abcam

Prices $373.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone TO-5

Anti-CANX / Calnexin Antibody (clone C8.B6) - LS-C21135 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone C8.B6

Anti-CANX / Calnexin Antibody (aa575-593) - LS-C21136 from LifeSpan Bioscience

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CANX / Calnexin Antibody (aa50-68) - LS-C21137 from LifeSpan Bioscience

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CANX / Calnexin Antibody - LS-C21138 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
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