![All lanes : Anti-Calnexin antibody [EPR3632] (ab92573) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HepG2 cell lysates Lysates/proteins at 10 µg per lane.Secondarystandard HRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19567_Calnexin--ER-membrane-marker-Primary-antibodies-ab92573-1.JPG)
All lanes : Anti-Calnexin antibody [EPR3632] (ab92573) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HepG2 cell lysates Lysates/proteins at 10 µg per lane.Secondarystandard HRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin embedded Human tonsil tissue using ab92573 at a 1/100 dilution.
Immunocytochemistry/Immunofluorescence analysis of human lung carcinoma A549 cells labelling Calnexin with ab92573. Cells were fixed with 4% PFA in PBS for 15 minutes washed three times with PBS, and then washed twice with 25 mM glycine–PBS. The cells were treated with cold methanol and washed three times with 0.3% Triton X-100 in PBS for permeabilization. The fixed cells were blocked with 5% BSA in PBS overnight. An Alexa Fluor® 594-conjugated anti-rabbit was used as the secondary antibody.