Anti-Calpain 10 antibody
| Name | Anti-Calpain 10 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28226 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide based on the aminoterminal end of domain-III in the large subunit, using the human sequence |
| Description | Rabbit Polyclonal |
| Gene | CAPN10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab28226 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28226, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab28226 staining in normal human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28226, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Calpain 10 is required for cell viability and is decreased in the aging kidney. - Calpain 10 is required for cell viability and is decreased in the aging kidney.
Covington MD, Arrington DD, Schnellmann RG. Am J Physiol Renal Physiol. 2009 Mar;296(3):F478-86. doi:
Calpain expression and activity during lens fiber cell differentiation. - Calpain expression and activity during lens fiber cell differentiation.
De Maria A, Shi Y, Kumar NM, Bassnett S. J Biol Chem. 2009 May 15;284(20):13542-50.
Mitochondrial calpain 10 activity and expression in the kidney of multiple - Mitochondrial calpain 10 activity and expression in the kidney of multiple
Giguere CJ, Covington MD, Schnellmann RG. Biochem Biophys Res Commun. 2008 Feb 1;366(1):258-62. Epub 2007 Dec 3.