CAPN10

Gene Symbol CAPN10
Entrez Gene 11132
Alt Symbol CANP10, NIDDM1
Species Human
Gene Type protein-coding
Description calpain 10
Other Description calcium-activated neutral proteinase 10|calpain-10|calpain-like protease CAPN10
Swissprots Q9HC92 Q96IG4 Q8NCD4 Q9HC96 Q9HC93 Q96JI2 Q9HC89 Q9HC95 Q9HC90 A8MVS7 Q9HC91 Q9HC94 Q4ZFV1
Accessions AAX88944 EAW71197 EAW71199 EAW71204 EAW71205 EAW71206 Q9HC96 AB058748 BAB47474 AF089088 AAG17966 AF089090 AAG17968 AF089091 AAG17969 AF089092 AAG17970 AF089093 AAG17971 AF089094 AAG17972 AF089096 AAG17974 AK027330 AK074807 BAC11220 AK128552 AK300279 BAH13249 AL558905 AU310002 BC004260 AAH04260 BC007553 AAH07553 BC107088 BI909439 BQ052841 BQ962770 DA413480 DA623373 DB224752 DQ893519 ABM84445 DQ896503 ABM87502 NM_023083 NP_075571 NM_023085 NP_075573 NM_021251 NM_023084 NM_023086 NM_023087 NM_023088 NM_023089
Function Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. May play a role in insulin-stimulated glucose uptake. {ECO:0000269|PubMed:17572128}.
Tissue Specificity Detected in primary skeletal muscle cells (at protein level). Ubiquitous. {ECO:0000269|PubMed:17572128}.

Calpain 10 (C-20) - sc-48454 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calpain 10 (C-20): sc-48454. Western blot analysis of Calpain 10 expression in 293T whole cell lysate.

Calpain 10 (H-50) - sc-134562 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Calpain 10 (C-14) - sc-48453 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Calpain 10 antibody - ab28226 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab28226 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28226, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Calpain 10 antibody - ab28220 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab28220 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28220, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Calpain 10 antibody - ab28222 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab28222 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28222, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Calpain 10 antibody - ab87206 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Calpain 10 antibody (ab87206) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Calpain 10 antibody - ab151312 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Calpain 10 antibody (ab151312) at 1/1000 dilution + HeLa cell lysate at 30 µg

Anti-Calpain 10 antibody - ab168995 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-Calpain 10 antibody (ab168995) at 1/500 dilutionLane 1 : Calpain 10 transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 15 µl per lane.

Anti-Calpain 10 antibody - Aminoterminal end domain T - ab38965 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CAPN10 / Calpain 10 Antibody - LS-C127191 from LifeSpan Bioscience

Prices $620.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CAPN10 / Calpain 10 Antibody - LS-C309715 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CAPN10 / Calpain 10 Antibody (N-Terminus) - LS-C102425 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CAPN10 / Calpain 10 antibody LS-C102425 Western blot of HT1080 cell lysate.

Anti-CAPN10 / Calpain 10 Antibody (aa44-93) - LS-C146420 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CAPN10 / Calpain 10 antibody LS-C146420 Western blot of PANC1 Cell lysate. Antibody concentration 1 ug/ml.

Calpain 10 Antibody - NBP1-87489 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Calpain 10 Antibody [NBP1-87489] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Calpain 10 Antibody - NBP1-53117 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Calpain 10 Antibody [NBP1-53117] - HT1080 cell lysate, concentration 0.2-1 ug/ml.

Calpain 10 Antibody - NBP1-33732 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Calpain 10 Antibody [NBP1-33732] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.

Calpain 10 Antibody - H00011132-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: Calpain 10 Antibody [H00011132-B01] - analysis of CAPN10 expression in transfected 293T cell line by CAPN10 MaxPab polyclonal antibody (B01). Lane 1: CAPN10 transfected lysate (74.90 KDa). Lane 2: Non-transfected lysate.

Calpain 10 Antibody - H00011132-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Calpain 10 Antibody [H00011132-D01P] - Analysis of CAPN10 expression in transfected 293T cell line by CAPN10 polyclonal antibody.Lane 1: CAPN10 transfected lysate(74.90 KDa).Lane 2: Non-transfected lysate.

Anti-CAPN10 antibody produced in rabbit - HPA004170 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CAPN10: <B>Cat. No. HPA004170</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate tissue showing granular cytoplasmic staining of glandular cells.

Anti-Calpain-10 (Domain II, Catalytic Domain), Large Subunit antibody produced in rabbit - C2489 from Sigma-Aldrich

Prices $324.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Calpain-10 (Domain T, N-Terminal), Large Subunit antibody produced in rabbit - C2864 from Sigma-Aldrich

Prices $324.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CAPN10, (N-terminal) antibody produced in rabbit - SAB2103459 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CAPN10, antibody produced in rabbit: <B>Cat. No. SAB2103459</B>: Immunoblotting analysis of CAPN10 in HT1080 cell lysate with CAPN10 anitbody at 1 μg/mL.

CAPN10 antibody - N-terminal region (ARP52404_P050) - ARP52404_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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