Anti-Calpain 2 antibody
| Name | Anti-Calpain 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39165 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide based on the amino terminal end of domain-III in the large subunit of Rat Calpain 2 |
| Description | Rabbit Polyclonal |
| Gene | CAPN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Calpain 2 antibody (ab39165) at 1/1000 dilutionLane 1 : Calpain 2 controlLane 2 : Cell lysates from human chondrosarcoma (treated with IL1 alpha) at 10 µgPerformed under reducing conditions.
ab39164 (4µg/ml) staining CALPAIN2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal - Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal
Subramanian V, Moorleghen JJ, Balakrishnan A, Howatt DA, Chishti AH, Uchida HA. PLoS One. 2013 Aug 19;8(8):e72214.
Calpains mediate epithelial-cell death during mammary gland involution: - Calpains mediate epithelial-cell death during mammary gland involution:
Arnandis T, Ferrer-Vicens I, Garcia-Trevijano ER, Miralles VJ, Garcia C, Torres L, Vina JR, Zaragoza R. Cell Death Differ. 2012 Sep;19(9):1536-48.
Hepatocellular carcinomas in B6C3F1 mice treated with Ginkgo biloba extract for - Hepatocellular carcinomas in B6C3F1 mice treated with Ginkgo biloba extract for
Hoenerhoff MJ, Pandiri AR, Snyder SA, Hong HH, Ton TV, Peddada S, Shockley K, Witt K, Chan P, Rider C, Kooistra L, Nyska A, Sills RC. Toxicol Pathol. 2013 Aug;41(6):826-41.
A cell spot microarray method for production of high density siRNA transfection - A cell spot microarray method for production of high density siRNA transfection
Rantala JK, Makela R, Aaltola AR, Laasola P, Mpindi JP, Nees M, Saviranta P, Kallioniemi O. BMC Genomics. 2011 Mar 28;12:162.
Fluid shear stress and sphingosine 1-phosphate activate calpain to promote - Fluid shear stress and sphingosine 1-phosphate activate calpain to promote
Kang H, Kwak HI, Kaunas R, Bayless KJ. J Biol Chem. 2011 Dec 9;286(49):42017-26.
Rapidly progressive diaphragmatic weakness and injury during mechanical - Rapidly progressive diaphragmatic weakness and injury during mechanical
Jaber S, Petrof BJ, Jung B, Chanques G, Berthet JP, Rabuel C, Bouyabrine H, Courouble P, Koechlin-Ramonatxo C, Sebbane M, Similowski T, Scheuermann V, Mebazaa A, Capdevila X, Mornet D, Mercier J, Lacampagne A, Philips A, Matecki S. Am J Respir Crit Care Med. 2011 Feb 1;183(3):364-71. doi:
Non-steroidal anti-inflammatory drugs inhibit calpain activity and membrane - Non-steroidal anti-inflammatory drugs inhibit calpain activity and membrane
Silver K, Leloup L, Freeman LC, Wells A, Lillich JD. Int J Biochem Cell Biol. 2010 Dec;42(12):2030-6. doi:
Calpain expression and activity during lens fiber cell differentiation. - Calpain expression and activity during lens fiber cell differentiation.
De Maria A, Shi Y, Kumar NM, Bassnett S. J Biol Chem. 2009 May 15;284(20):13542-50.
Mitochondrial m-calpain plays a role in the release of truncated - Mitochondrial m-calpain plays a role in the release of truncated
Ozaki T, Yamashita T, Ishiguro S. Biochim Biophys Acta. 2009 Dec;1793(12):1848-59. doi:
Asymmetric localization of calpain 2 during neutrophil chemotaxis. - Asymmetric localization of calpain 2 during neutrophil chemotaxis.
Nuzzi PA, Senetar MA, Huttenlocher A. Mol Biol Cell. 2007 Mar;18(3):795-805. Epub 2006 Dec 27.