CAPN2

Gene Symbol CAPN2
Entrez Gene 824
Alt Symbol CANP2, CANPL2, CANPml, mCANP
Species Human
Gene Type protein-coding
Description calpain 2, (m/II) large subunit
Other Description CANP 2|M-calpain|calcium-activated neutral proteinase 2|calpain 2, large [catalytic] subunit|calpain 2, large subunit|calpain M-type|calpain large polypeptide L2|calpain, large polypeptide L2|calpain-2 catalytic subunit|calpain-2 large subunit|millimolar-calpain
Swissprots Q8WU26 A6NDG7 Q16738 E7ES58 Q9HBB1 B7ZA96 P17655 Q6PJT3
Accessions AAA52760 AAV80421 EAW93249 EAW93250 EAW93251 P17655 AB209855 BAD93092 AF261089 AAF99682 AI089162 AK023851 AK055046 AK056838 AK097247 BAG53441 AK124751 BAC85936 AK297800 BAG60139 AK303952 BAG64879 AK315179 BAG37620 AK316211 BAH14582 BC007686 AAH07686 BC011828 AAH11828 BC021303 AAH21303 DA448245 DQ893312 ABM84238 DQ893620 ABM84546 EU176804 ABW03605 M23254 AAA35645 XM_011544286 XP_011542588 NM_001146068 NP_001139540 NM_001748 NP_001739
Function Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). {ECO:0000269|PubMed:17650508}.
Subcellular Location Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding.
Tissue Specificity Ubiquitous.
Top Pathways Apoptosis, Focal adhesion, Alzheimer's disease, Protein processing in endoplasmic reticulum

Calpain 2 Large Subunit (M-type) Antibody - 2539 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C2C12 and PC3 cells, using Calpain 2 Large Subunit (M-type) Antibody.

Calpain (B-8) - sc-271856 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain (B-8): sc-271856. Western blot analysis of Calpain expression in HeLa whole cell lysate.

Calpain (H-240) - sc-30064 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Calpain (H-240): sc-30064. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Calpain 2 (E-10) - sc-373966 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 2 (E-10): sc-373966. Western blot analysis of Calpain 2 expression in A-431 (A) and HeLa (B) whole cell lysates.

Calpain 2 (N-19) - sc-7533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calpain 2 (N-19): sc-7533. Western blot analysis of Calpain 2 expression in A-431 whole cell lysate.

Calpain 2 (F-11) - sc-373967 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Calpain 2 (F-11): sc-373967. Western blot analysis of Calpain 2 expression in A-431 (A) and HeLa (B) whole cell lysates.

Calpain 2 (C-19) - sc-7532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calpain 2 (C-19): sc-7532. Western blot analysis of Calpain 2 expression in TF-1 (A), K-562 (B), A-431 (C), T-47D (D) and PC-3 (E) whole cell lysates.

Anti-Calpain 2 antibody - ab39165 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Calpain 2 antibody (ab39165) at 1/1000 dilutionLane 1 : Calpain 2 controlLane 2 : Cell lysates from human chondrosarcoma (treated with IL1 alpha) at 10 µgPerformed under reducing conditions.

Anti-Calpain 2 antibody - ab39168 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Calpain 2 antibody - ab155666 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Calpain 2 antibody (ab155666) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

Anti-Calpain 2 antibody - ab88110 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Calpain 2 antibody (ab88110) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg/ml per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Calpain 2 antibody - ab39167 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab39167 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Calpain 2 antibody - ab28259 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Calpain 2 antibody (ab28259) at 1/1000 dilutionLane 1 : Calpain controlLane 2 : Cell lysates from human chodrosarcoma (treated with II1a)

Anti-Calpain 2 antibody - ab182603 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Calpain 2 antibody (ab182603) at 1/400 dilutionLane 1 : mouse brain tissue lysateLane 2 : 293T cell lysateLane 3 : mouse skin tissue lysateLysates/proteins at 30 µg per lane.SecondaryDonkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilutiondeveloped using the ECL technique

Anti-Calpain 2 antibody - ab39169 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Calpain 2 antibody - ab135277 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Calpain 2 with ab135277 at 1/50.

Anti-Calpain 2 antibody [107-82] - ab3588 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 107-82
Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Calpain 2 (green) with ab3588 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

Anti-Calpain 2 antibody [1E8] - ab713 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E8
Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.

Anti-Calpain 2 antibody [3G1] - ab139158 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3G1
All lanes : Anti-Calpain 2 antibody [3G1] (ab139158) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY Calpain 2 cDNALysates/proteins at 5 µg per lane.

Anti-Calpain 2 antibody [EPR2562Y] - ab75994 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2562Y
Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/500 dilution + HeLa cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilutionObserved band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-Calpain 2 antibody [EPR5977] - ab126600 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5977
All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilutionLane 1 : PC3 cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-CAPN2 / Calpain 2 / M-Calpain Antibody (C-Terminus) - LS-C48359 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CAPN2 / Calpain 2 / M-Calpain Antibody (aa502-699, clone 107-82) - LS-C84831 from LifeSpan Bioscience

Prices $800.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 107-82

Anti-CAPN2 / Calpain 2 / M-Calpain Antibody (aa305-354) - LS-C199783 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CAPN2 / Calpain 2 / M-Calpain Antibody (Internal, clone EPR2562Y) - LS-C105315 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2562Y
Western blot analysis on HeLa cell lysate using anti-Calpain2 antibody (LS-C105315), dilution 1:500.
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