Anti-Calponin antibody [CALP]

• Supplier: Abcam
• Catalog: ab700
• Prices: $395.00
• Sizes: 500 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: CALP
• Applications: IHC-F FC ICC/IF ICC/IF IHC-F IHC-P
• Species Reactivities: Rat, Human
• Antigen: Crude human uterus extract
• Description: Mouse Monoclonal
• Gene: CNN1
• Conjugate: Unconjugated

Product images

ab700 at a 1/50 dilution staining rat heart tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed prior to incubation with the antibody overnight at 4°C. An Alex-Fluor ® 488 conjugated  goat anti-mouse antibody was used as the secondary. The image was taken  with a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode and shows stained Calponin (smooth muscle cells-green) and counterstained nuclei (DAPI-blue) (left panel).DIC (phase) image of the same tissue (right panel) confirm the localization of the calponin staining in the arterial smooth muscle in the heart tissue with very little background.See Abreview

ab700 staining Calponin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Alexa Fluor®488-conjugated chicken polyclonal to mouse IgG was used as secondary antibody at 1/400 dilution. Image shows combined fluorescent and transmitted light images. Hoechst 33342 was used to stain the cell nuclei (blue).See Abreview

Product References

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