Anti-Calponin antibody [EP798Y]

• Supplier: Abcam
• Catalog: ab46794
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP798Y
• Applications: WB IHC-P ICC/IF ICC/IF IHC-F ICC/IF
• Species Reactivities: Mouse, Rat, Dog, Human, Pig
• Antigen: Synthetic peptide corresponding to residues near the C-term of Calponin (Human)
• Description: Rabbit Monoclonal
• Gene: CNN1
• Conjugate: Unconjugated

Product images

Anti-Calponin antibody [EP798Y] (ab46794) at 1/20000 dilution + Human bladder lysate at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilutionObserved band size : 34 kDa (why is the actual band size different from the predicted?)

Immunofluorescent staining of Hela cells using ab46794 at 1/100 dilution

Immunohistochemical staining of paraffin-embedded human smooth muscle using ab46794 at 1/100 dilution

ab46794 staining Calponin in porcine aortic smooth muscle cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100. Samples were then incubated with primary antibody at 1/50 for 1 hour at 25°C. The secondary antibody used was ab6717 Goat polyclonal to Rabbit IgG - H&L (FITC) (green) used at a 1/400 dilution.See Abreview

ICC/IF image of ab46794 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46794, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-Calponin antibody [EP798Y] (ab46794) at 1/20000 dilutionLane 1 : Whole tissue lysate prepared from bovine lymph vesselsLane 2 : Whole tissue lysate prepared from bovine lymph vesselsLysates/proteins at 10 µg per lane.SecondaryHRP conjugated goat anti-rabbit polyclonal at 1/5000 dilutiondeveloped using the ECL techniqueObserved band size : 34 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteImage courtesy of an anonymous Abreview.See Abreview

ab46794 showing positive staining in Normal kidney vessels tissue.

ab46794 showing positive staining in Normal lung vessel tissue.

ab46794 showing positive staining in Normal tonsil vessel tissue.

ab46794 showing positive staining in Normal uterus tissue.

ab46794 showing negative staining in Skeletal muscle tissue.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

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