Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling Calponin with ab175572 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Anti-Calponin antibody - N-terminal (ab175572) at 1/1000 dilution + NCI-H460 cell line lysates at 35 µg
Flow Cytometric analysis of NCI-H292 cells labeling Calponin with ab175572 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.