All lanes : Anti-CaMKII beta antibody (ab107163) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLane 4 : Forebrain (Mouse) Tissue Lysate Lane 5 : Rat Cortex Tissue LysateLane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 7 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.