Anti-CAMKIV antibody [EP2565AY]

• Supplier: Abcam
• Catalog: ab68218
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP2565AY
• Applications: ICC/IF ICC/IF WB IP IHC-P FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues near the C-terminus of human CAMKIV
• Description: Rabbit Monoclonal
• Gene: CAMK4
• Conjugate: Unconjugated

Product images

ab68218 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68218 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-CAMKIV antibody [EP2565AY] (ab68218) at 1/1000000 dilutionLane 1 : Jurkat cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab68218 at 1/250-1/500 dilution staining CAMKIV in human brain tissue sections.

ab68218 at 1/250-1/500 dilution staining CAMKIV in human ovarian adenocarcinoma tissue sections.