Anti-cAMP antibody [M486]
| Name | Anti-cAMP antibody [M486] |
|---|---|
| Supplier | Abcam |
| Catalog | ab70280 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | M486 |
| Applications | IHC-F ICC/IF ICC/IF WB ELISA |
| Antigen | A chemically linked 3', 5'-Cyclic Adenosine Monophosphate (cAMP) |
| Description | Mouse Monoclonal |
| Gene | CHAMP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ELISA Plot:. Adenosine-3', 5'-cyclic AMP immobilized onto plates, followed by addition of stand cyclic AMP. The mouse anti c-AMP was added subsequentially, and visiualized by chromatogenic substrate. Each sample was done in triplicate. IC50 was then calculated.
ICC/IF image of ab70280 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab70280 staining cAMP in HEK293 cells treated with neuropeptide S (ab120174), by ICC/IF. Increase in cAMP expression correlates with increased concentration of neuropeptide S, as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120174 (neuropeptide S) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70280 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab70280 staining cAMP in HEK293 cells treated with neuropeptide S (ab120246), by ICC/IF. Increase in cAMP expression correlates with increased concentration of neuropeptide S, as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120246 (neuropeptide S) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70280 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Product References
Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival - Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival
Corredor RG, Trakhtenberg EF, Pita-Thomas W, Jin X, Hu Y, Goldberg JL. J Neurosci. 2012 May 30;32(22):7734-44.
Dopamine and full-field illumination activate D1 and D2-D5-type receptors in - Dopamine and full-field illumination activate D1 and D2-D5-type receptors in
Ogata G, Stradleigh TW, Partida GJ, Ishida AT. J Comp Neurol. 2012 Dec 1;520(17):4032-49.