CHAMP1

Gene Symbol CHAMP1
Entrez Gene 283489
Alt Symbol C13orf8, CAMP, CHAMP, ZNF828
Species Human
Gene Type protein-coding
Description chromosome alignment maintaining phosphoprotein 1
Other Description chromosome alignment-maintaining phosphoprotein 1|zinc finger protein 828
Swissprots Q6P181 Q9BST0 Q8NC88 B3KU06 Q96JM3
Accessions EAX09253 EAX09254 Q96JM3 AB058705 BAB47431 AK074894 BAC11273 AK096346 BAG53268 BC004820 AAH04820 BC019576 BC065237 AAH65237 BM663717 CN365659 DA289328 DB229502 EU446515 ABZ92044 NM_001164144 NP_001157616 NM_001164145 NP_001157617 NM_032436 NP_115812
Function Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore. {ECO:0000269|PubMed:21063390}.
Subcellular Location Nucleus {ECO:0000269|PubMed:21063390}. Chromosome {ECO:0000269|PubMed:21063390}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:21063390}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:21063390}.

ZNF828 (C-15) - sc-84040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

ZNF828 (D-17) - sc-84041 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

ZNF828 (H-95) - sc-292297 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ZNF828 (H-95): sc-292297. Western blot analysis of ZNF828 expression in U-251-MG whole cell lysate.

ZNF828 (E-22) - sc-134212 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
ZNF828 (E-22): sc-134212. Western blot analysis of ZNF828 expression in K-562 whole cell lysate.

Anti-C13orf8 antibody - ab48703 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-C13orf8 antibody (ab48703) at 0.25 µg/ml + Daudi cell lysate at 10 µg with 5% skimmed milk in PBSSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-C13orf8 antibody - ab169249 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-C13orf8 antibody (ab169249) at 1 µg/mlLane 1 : C13orf8 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-C13orf8 antibody - ab173486 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-C13orf8 antibody (ab173486) at 1/100 dilution + MCF7 cell line lysate at 35 µg

Anti-cAMP antibody - ab832 from Abcam

Prices $370.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal

Anti-cAMP antibody [EP8471] - ab134901 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP8471
Competitive inhibition ELISA using ab134901. 50µl of cAMP-SPDP-BSA was coated in 96-wells. After 1% BSA blocking, 25 µl cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, H2O and 25 µl of ab134901 were added. HRP conjugated goat anti-rabbit IgG-Fc-HR antibody was used to develop the color.

Anti-cAMP antibody [M486] - ab70280 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone M486
ELISA Plot:. Adenosine-3', 5'-cyclic AMP immobilized onto plates, followed by addition of stand cyclic AMP. The mouse anti c-AMP was added subsequentially, and visiualized by chromatogenic substrate. Each sample was done in triplicate. IC50 was then calculated.

Anti-cAMP antibody [SPM486] - ab24851 from Abcam

Prices $390.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone SPM486
ab24851 staining cAMP in MALME-3M cells treated with melanotan II (ab141161), by ICC/IF. Increase of cAMP expression correlates with increased concentration of melanotan II, as described in literature.The cells were incubated at 37°C for 4h in media containing different concentrations of ab141161 (melanotan II) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab24851 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-C13orf8 / ZNF828 Antibody (aa203-229) - LS-C157515 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ZNF828 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The ZNF828 antibody detected the ZNF828 protein (arrow).

Anti-C13orf8 / ZNF828 Antibody (aa1-50) - LS-C290857 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse CHAMP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CHAMP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-C13orf8 / ZNF828 Antibody (aa203-229, APC) - LS-C243524 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C13orf8 / ZNF828 Antibody (aa203-229, AP) - LS-C243525 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C13orf8 / ZNF828 Antibody (aa203-229, FITC) - LS-C243527 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C13orf8 / ZNF828 Antibody (aa203-229, PE) - LS-C243529 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C13orf8 / ZNF828 Antibody (aa203-229, Azide-free, HRP) - LS-C247408 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C13orf8 / ZNF828 Antibody (aa202-229) - LS-C326718 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C13orf8 / ZNF828 Antibody - LS-C306610 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-C13orf8 / ZNF828 Antibody (aa261-310) - LS-C31428 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ZNF828 / C13orf8 antibody LS-C31428 was used in IHC to stain formalin-fixed, paraffin-embedded human muscle.

Anti-C13orf8 / ZNF828 Antibody (aa762-812) - LS-C290859 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse CHAMP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CHAMP1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-C13orf8 / ZNF828 Antibody (aa203-229, Biotin) - LS-C243526 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

ZNF828 Antibody - NBP1-88403 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: ZNF828 Antibody [NBP1-88403] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli.

ZNF828 Antibody - NBP1-88402 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: ZNF828 Antibody [NBP1-88402] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
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