Anti-Cannabinoid Receptor II antibody

• Supplier: Abcam
• Catalog: ab3561
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F WB IHC-P ICC/IF ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human, Hamster
• Antigen: Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal)
• Description: Rabbit Polyclonal
• Gene: CNR2
• Conjugate: Unconjugated

Product images

ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab3561 at 1/5000 staining Rat spinal cord tissue sections by IHC-P. Following nerve trans-section, the animal was transcardially perfused with PBS followed by 4% formaldehyde (no post fixation) then frozen in OCT and stored at -80°C until required. 20µm sections were cut and stained with the antibody for 18 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.See Abreview

ab3561 staining Cannabinoid Receptor II in murine BV2 cells by Immunocytochemistry/ Immunofluorescence. Cells were stimulated with 10 uM ADP for 15 minutes at 37°C, 5%CO2 then washed 3X with PBS and fixed with 2% formaldehyde for 15 minutes at room temperature. A blocking step was performed for 1 hour at room temperature with 5% FBS/PBS/0.01% Triton-X-100. ab3561 used at a 1/500 dilution for 1 hour at room temperature then washed 3X PBS. The secondary was an Alexa Fluor 488 conjugated antibody, used at a 1/250 for 1 hour at room temperature, washed 3X PBS then mounted in Vectashield containing DAPI.

All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilutionLane 1 : HT29 cell lysateLane 2 : C6 cell lysateLane 3 : Rat colon cell lysateLysates/proteins at 25 µg per lane.

Quantification of CB1 and CB2 receptor surface expression in primary Human and HaCaT keratinocytes. (A) FL-1 shows autofluorescence (black population), non-specific binding of secondary antibody (dark grey population) and specific immunofluorescence obtained with CB1 (ab3558) and CB2 (ab3561) receptor antibodies, respectively (light grey population). Histogram shows representative measurement (5000 cells counted). (B) Differences in CB receptor expression between primary and HaCaT keratinocytes, showing marked increased expression of CB2 in primary keratinocytes (geo mean corrected for non-specific binding of secondary Ab). Data show mean values ±SEM obtained with two human primary keratinocyte samples and HaCaT cells, each measured three times.

Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.

Product References

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