Anti-Cannabinoid Receptor II antibody
| Name | Anti-Cannabinoid Receptor II antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45942 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Cannabinoid Receptor II |
| Description | Rabbit Polyclonal |
| Gene | CNR2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cannabinoid Receptor II antibody (ab45942) at 1 µg/mlLane 1 : Spinal Cord (Human) Tissue LysateLane 2 : Spinal Cord (Rat) Tissue Lysate Lane 3 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab45942 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45942, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Excitability of prefrontal cortical pyramidal neurons is modulated by activation - Excitability of prefrontal cortical pyramidal neurons is modulated by activation
den Boon FS, Chameau P, Schaafsma-Zhao Q, van Aken W, Bari M, Oddi S, Kruse CG, Maccarrone M, Wadman WJ, Werkman TR. Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3534-9. doi:
Differential expression of functional cannabinoid receptors in human bladder - Differential expression of functional cannabinoid receptors in human bladder
Tyagi V, Philips BJ, Su R, Smaldone MC, Erickson VL, Chancellor MB, Yoshimura N, Tyagi P. J Urol. 2009 Apr;181(4):1932-8.