
Immunofluorescent analysis of ice cold methanol-fixed A431 cells either untreated (left) or treated with 200 µm CoCl2 for 48 hours (right), labeling Carbonic Anhydrase IX with ab184630 at 1/100 dilution. Counterstained with Hoechst 33342 (lower images)
![All lanes : Anti-Carbonic Anhydrase IX antibody [GT14212] (ab184630) at 1/1000 dilutionLane 1 : HeLa whole cell lysate/extract (untreated)Lane 2 : HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 24 hr)Lane 3 : HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 48 hr)Lane 4 : HeLa whole cell lysate/extract (untreated)Lane 5 : HeLa whole cell lysate/extract (1% O2 treatment for 24 hr)Lane 6 : HeLa whole cell lysate/extract (1% O2 treatment for 48 hr)Lysates/proteins at 30 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/20491_ab184630-215550-ab184630.jpg)
All lanes : Anti-Carbonic Anhydrase IX antibody [GT14212] (ab184630) at 1/1000 dilutionLane 1 : HeLa whole cell lysate/extract (untreated)Lane 2 : HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 24 hr)Lane 3 : HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 48 hr)Lane 4 : HeLa whole cell lysate/extract (untreated)Lane 5 : HeLa whole cell lysate/extract (1% O2 treatment for 24 hr)Lane 6 : HeLa whole cell lysate/extract (1% O2 treatment for 48 hr)Lysates/proteins at 30 µg per lane.