CA9

Gene Symbol CA9
Entrez Gene 768
Alt Symbol CAIX, MN
Species Human
Gene Type protein-coding
Description carbonic anhydrase IX
Other Description CA-IX|P54/58N|RCC-associated antigen G250|RCC-associated protein G250|carbonate dehydratase IX|carbonic anhydrase 9|carbonic dehydratase|membrane antigen MN|pMW1|renal cell carcinoma-associated antigen G250
Swissprots Q5T4R1 Q16790
Accessions CBL94025 EAW58359 Q16790 AI023541 AJ010588 CAB82444 AL554705 BC014950 AAH14950 DQ892208 ABM83134 DQ895404 ABM86330 EF122496 ABL67717 X66839 CAA47315 XM_006716869 XP_006716932 XM_006716870 XP_006716933 XR_428428 NM_001216 NP_001207
Function Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. {ECO:0000269|PubMed:18703501}.
Subcellular Location Nucleus {ECO:0000269|PubMed:8486430}. Nucleus, nucleolus {ECO:0000269|PubMed:8486430}. Cell membrane {ECO:0000269|PubMed:8486430}; Single-pass type I membrane protein {ECO:0000269|PubMed:8486430}. Cell projection, microvillus membrane {ECO:0000269|PubMed:8486430}; Single-pass type I membrane protein {ECO:0000269|PubMed:8486430}. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Tissue Specificity Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Top Pathways Nitrogen metabolism

CA9 (D10C10) Rabbit mAb - 5648 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10C10
Western blot analysis of total extracts from LN18 and SW620 cells using CA9 (D10C10) Rabbit mAb.

CA9 (D47G3) Rabbit mAb - 5649 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D47G3
Western blot analysis of extracts from LN18 and SW620 cells using CA9 (D47G3) Rabbit mAb.

CA IX (H-11) - sc-365900 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CA IX (H-11): sc-365900. Western blot analysis of CA IX expression in HeLa whole cell lysate.

CA IX (H-120) - sc-25599 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CA IX (H-120): sc-25599. Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

CA IX (N-19) - sc-17253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CA IX (N-19): sc-17253. Western blot analysis of CA IX expression in PMA-treated HeLa (A), F9 (B) and LS1034 (C) whole cell lysates.

Anti-Carbonic Anhydrase IX antibody - ab184006 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Carbonic Anhydrase IX antibody (ab184006) at 1/1000 dilutionLane 1 : HepG2 whole cell lysate, untreatedLane 2 : HepG2 whole cell lysate, treated with 200 µM CoCl2 for 24 hrLysates/proteins at 30 µg per lane.

Anti-Carbonic Anhydrase IX antibody - ab10471 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Carbonic Anhydrase IX antibody (ab10471) at 1 µg/ml + Carbonic Anhydrase IX recombinant protein at 0.1 µg/mlSecondaryGoat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilutionPerformed under reducing conditions.

Anti-Carbonic Anhydrase IX antibody - ab197381 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue, labeling Carbonic Anhydrase IX with ab197381 at a 1/50 dilution.

Anti-Carbonic Anhydrase IX antibody - ab128883 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of Carbonic Anhydrase IX staining in Human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab128882, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Carbonic Anhydrase IX antibody - ab15086 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin fixed paraffin embedded renal carcinoma tissue, positively stained using Ab15086.

Anti-Carbonic Anhydrase IX antibody [10F7A8] - N-terminal - ab181464 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10F7A8
All lanes : Anti-Carbonic Anhydrase IX antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilutionLane 1 : A431 cell line lysateLane 2 : SW620 cell line lysate

Anti-Carbonic Anhydrase IX antibody [2D3] - ab107257 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D3
All lanes : Anti-Carbonic Anhydrase IX antibody [2D3] (ab107257) at 1/500 dilutionLane 1 : Hela Cell lysateLane 2 : A549 Cell Lysate

Anti-Carbonic Anhydrase IX antibody [EPR4151(2)] - ab108351 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4151(2)
All lanes : Anti-Carbonic Anhydrase IX antibody [EPR4151(2)] (ab108351) at 1/1000 dilutionLane 1 : HT-29 cell lysateLane 2 : Human stomach lysateLysates/proteins at 10 µg per lane.

Anti-Carbonic Anhydrase IX antibody [GT14212] - ab184630 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT14212
Immunofluorescent analysis of ice cold methanol-fixed A431 cells either untreated (left) or treated with 200 µm CoCl2 for 48 hours (right), labeling Carbonic Anhydrase IX with ab184630 at 1/100 dilution. Counterstained with Hoechst 33342 (lower images)

Anti-Carbonic Anhydrase IX antibody [MM0610-3B15] - ab135159 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0610-3B15

Anti-Carbonic Anhydrase IX antibody [SP106] - ab105226 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP106
Flow cytometric analysis of rabbit anti-Carbonic Anhydrase IX (SP106) antibody ab105226 (1/100) in HT29  cells (green) compared to negative control of rabbit IgG (blue).

Anti-Renal Cell Carcinoma antibody [66.4.C2/PN-15] - ab187365 from Abcam

Prices $370.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 66.4.C2/PN-15
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissue, labeling Renal Cell Carcinoma using ab187365 at 1 µg/ml.

Anti-CA9 / Carbonic Anhydrase IX Antibody (aa37-186, clone 10F7A8) - LS-C285840 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 10F7A8
Western blot using CA9 monoclonal antibody against HEK293 (1) and CA9 (AA: 37-186)-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CA9 / Carbonic Anhydrase IX Antibody (aa37-186, clone 7C4A5) - LS-C285841 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7C4A5
IHC of paraffin-embedded rectum cancer tissues using ZEB1 mouse monoclonal antibody with DAB staining.

Anti-CA9 / Carbonic Anhydrase IX Antibody (C-Terminus) IHC-plus™ - LS-B273 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CA9 / Carbonic Anhydrase IX antibody IHC of human kidney, renal cell carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B273 concentration 0.625 ug/ml.

Anti-CA9 / Carbonic Anhydrase IX Antibody (APC) - LS-C35269 from LifeSpan Bioscience

Prices $545.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal

Anti-CA9 / Carbonic Anhydrase IX Antibody (CF) - LS-C35271 from LifeSpan Bioscience

Prices $445.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal

Anti-CA9 / Carbonic Anhydrase IX Antibody (clone 303123, PE) - LS-C35270 from LifeSpan Bioscience

Prices $590.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal
Clone 303123

Anti-CA9 / Carbonic Anhydrase IX Antibody (C-Terminus, Biotin) - LS-C83728 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CA9 / Carbonic Anhydrase IX Antibody (C-Terminus) - LS-C77714 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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