Anti-CARD15 antibody [2D9]
| Name | Anti-CARD15 antibody [2D9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab31488 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2D9 |
| Applications | WB IP IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 28-301 (MCS QEAFQAQRSQ LVELLVSGSL EGFESVLDWL LSWEVLSWED YEGFHLLGQP LSHLARRLLD TVWNKGTWAC QKLIAAAQEA QADSQSPKLH GCWDPHSLHP ARDLQSHRPA IVRRLHSHVE NMLDLAWERG FVSQYECDEI RLPIFTPSQR ARRLLDLATV KANGLAAFLL QHVQELPVPL ALPLEAATCK KYMAKLRTTV SAQSRFLSTY DGAETLCLED IYTENVLEVW ADVGMAGPPQ KSPATLGLEE LFSTPGHLND DADTVLVVGE A) of Human CARD15 |
| Description | Mouse Monoclonal |
| Gene | NOD2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-CARD15 antibody [2D9] (ab31488) at 2 µg/ml + HEK 293 overexpressing CARD15 at 20 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/20683_CARD15-Primary-antibodies-ab31488-4.jpg)
Anti-CARD15 antibody [2D9] (ab31488) at 2 µg/ml + HEK 293 overexpressing CARD15 at 20 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of CARD15 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31488, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Expression and functional characterization of NOD2 in decidual stromal cells - Expression and functional characterization of NOD2 in decidual stromal cells
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miRNAs affect the expression of innate and adaptive immunity proteins in celiac - miRNAs affect the expression of innate and adaptive immunity proteins in celiac
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Effects of NOD-like receptors in human B lymphocytes and crosstalk between - Effects of NOD-like receptors in human B lymphocytes and crosstalk between
Petterson T, Jendholm J, Mansson A, Bjartell A, Riesbeck K, Cardell LO. J Leukoc Biol. 2011 Feb;89(2):177-87.
Organic dust augments nucleotide-binding oligomerization domain expression via an - Organic dust augments nucleotide-binding oligomerization domain expression via an
Poole JA, Kielian T, Wyatt TA, Gleason AM, Stone J, Palm K, West WW, Romberger DJ. Am J Physiol Lung Cell Mol Physiol. 2011 Sep;301(3):L296-306. doi: