Anti-CARD15 antibody
| Name | Anti-CARD15 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab36836 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2 |
| Blocking Peptide | Human CARD15 peptide |
| Description | Rabbit Polyclonal |
| Gene | NOD2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-CARD15 antibody (ab36836) at 2 µg/ml + HeLa cell lysate
ab36836 at 10µg/ml staining CARD15 in Hela cells by Immunocytochemistry.
IHC image of ab36836 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36836, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunofluorescence of NOD2 in HeLa cells using ab36836 at 20 ug/ml.