Flow cytometric analysis of Jurkat cells labeling CARD4 with ab170547 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of HepG2 cells labeling CARD4 with ab170547 at 1/10 dilution (green) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG. DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin fixed, paraffin embedded Human pancreas tissue labeling CARD4 with ab170547 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
All lanes : Anti-CARD4 antibody - C-terminal (ab170547) at 1/100 dilutionLane 1 : HL60 cell lysateLane 2 : CEM cell lysateLysates/proteins at 35 µg per lane.SecondaryAnti-rabbit HRP conjugated antibody at 1/10000 dilutiondeveloped using the ECL technique
Anti-CARD4 antibody - C-terminal (ab170547) at 1/100 dilution + Mouse bladder tissue lysate at 35 µgSecondaryAnti-rabbit HRP conjugated antibody at 1/10000 dilutiondeveloped using the ECL technique