Anti-CARD8 antibody
| Name | Anti-CARD8 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab24186 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF FC ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to amino acids within the last 50 amino acids of the C terminus of human CARD8, GenBank accession AAG50014 |
| Blocking Peptide | Human CARD8 peptide |
| Description | Rabbit Polyclonal |
| Gene | CARD8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-CARD8 antibody (ab24186) at 2 µg/ml (CARD8 expression in K562 cell lysate)Lane 2 : Anti-CARD8 antibody (ab24186) at 4 µg/ml (CARD8 expression in K562 cell lysate)Lane 1 : As aboveLane 2 : As above
ab24186 at 10µg/ml staining CARD8 in K562 cells by ICC/IF
ab24186 staining CARD8 in human monocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab24186 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.See Abreview
ab24186 staining CARD8 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in 2% BSA and blocked with 2% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody. WGA = Wheat Germ Agglutinin See Abreview
Product References
CARD8 and NLRP1 undergo autoproteolytic processing through a ZU5-like domain. - CARD8 and NLRP1 undergo autoproteolytic processing through a ZU5-like domain.
D'Osualdo A, Weichenberger CX, Wagner RN, Godzik A, Wooley J, Reed JC. PLoS One. 2011;6(11):e27396.
The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 - The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9
Mille F, Thibert C, Fombonne J, Rama N, Guix C, Hayashi H, Corset V, Reed JC, Mehlen P. Nat Cell Biol. 2009 Jun;11(6):739-46.
A genome-wide in vitro bacterial-infection screen reveals human variation in the - A genome-wide in vitro bacterial-infection screen reveals human variation in the
Ko DC, Shukla KP, Fong C, Wasnick M, Brittnacher MJ, Wurfel MM, Holden TD, O'Keefe GE, Van Yserloo B, Akey JM, Miller SI. Am J Hum Genet. 2009 Aug;85(2):214-27.