Lanes 1 - 2 : Anti-Casein Kinase 1 gamma 2 antibody (ab102554)Lanes 3 - 4 : Anti-Casein Kinase 1 gamma 2 antibody - C-terminal (ab70862) at 1/200 dilutionLanes 5 - 6 : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilutionLane 1 : U2OS cells transfected with a plasmid expressing FLAG-tagged CK1G2Lane 2 : U2OS cells transfected with a plasmid expressing FLAG onlyLane 3 : U2OS cells transfected with a plasmid expressing FLAG-tagged CK1G2Lane 4 : U2OS cells transfected with a plasmid expressing FLAG onlyLane 5 : U2OS cells transfected with a plasmid expressing FLAG-tagged CK1G2Lane 6 : U2OS cells transfected with a plasmid expressing FLAG onlyLysates/proteins at 90 µg per lane.SecondaryHRP- conjugated Goat anti-rabbit polyclonal at 1/10000 dilutiondeveloped using the ECL technique
Anti-Casein Kinase 1 gamma 2 antibody - C-terminal (ab70862) at 1/100 dilution + mouse muscle tissue lysate at 12.5 µgSecondaryHRP-anti-rabbit
Immunohistochemical staining of formalin-fixed, paraffin-embedded human hepatocarcinoma with ab70862 at a 1/10 dilution, followed by HRP-conjugated secondary antibody and DAB staining.