Anti-CASK antibody [S56A-50]
| Name | Anti-CASK antibody [S56A-50] |
|---|---|
| Supplier | Abcam |
| Catalog | ab99039 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S56A-50 |
| Applications | WB IP IHC-P IHC-F FC |
| Species Reactivities | Mouse, Rat, Human, Xenopus, Zebrafish |
| Antigen | Fusion protein: NSFYGDPPEE LPDFSEDPTS SGLLAAERAV SQVLDSLEEI HALTDCSEKD LDFLHSVFQD QHLHTLLDLY DKINTKSSPQ IRNPPSDAV, corresponding to amino acids 318-415 of Rat CASK (accession number Q62915) |
| Description | Mouse Monoclonal |
| Gene | CASK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab99039 staining CASK in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
![Anti-CASK antibody [S56A-50] (ab99039) at 1/1000 dilution + Rat brain membrane tissue lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21029_ab99039-199522-ab99039wb.jpg)
Anti-CASK antibody [S56A-50] (ab99039) at 1/1000 dilution + Rat brain membrane tissue lysate
![Overlay histogram showing SH-SY5Y cells stained with ab99039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab99039, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21030_ab99039-1-ab99039FC.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab99039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab99039, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.