All lanes : Anti-Caspase-10 antibody [EPR10890] (ab177475) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.
Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10 with ab177475 at 1/50 dilution.
Flow cytometric analysis of permeabilized K562 cells labeling Caspase 10 with ab177475 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).