Anti-Caspase-2 antibody [EPR16796]

• Supplier: Abcam
• Catalog: ab179520
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16796
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Mouse Caspase-2 aa 150-350
• Description: Rabbit Monoclonal
• Gene: CASP2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hoursLane 2 : Untreated HeLa whole cell lysateLane 3 : C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hoursLane 4 : Untreated C6 whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution + Human fetal kidney lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Caspase-2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179520 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab179520 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell extract. Lane 2: Rabbit monoclonal IgG (ab172730) instead of ab179520 in HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.