![All lanes : Anti-Caspase-2 antibody [EPR17880] (ab202420) at 1/10000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) treated with 25uM etoposide for 5 hours whole cell lysatesLane 2 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21157_ab202420-245131-ab202420-wb-1.jpg)
All lanes : Anti-Caspase-2 antibody [EPR17880] (ab202420) at 1/10000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) treated with 25uM etoposide for 5 hours whole cell lysatesLane 2 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
![All lanes : Anti-Caspase-2 antibody [EPR17880] (ab202420) at 1/2000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal kidney lysateLane 3 : Human thymus lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21158_ab202420-245130-ab202420-wb-2.jpg)
All lanes : Anti-Caspase-2 antibody [EPR17880] (ab202420) at 1/2000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal kidney lysateLane 3 : Human thymus lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
![All lanes : Anti-Caspase-2 antibody [EPR17880] (ab202420) at 1/2000 dilutionLane 1 : C6 (Rat glial tumor cells) cell lysateLane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21159_ab202420-245129-ab202420-wb-3.jpg)
All lanes : Anti-Caspase-2 antibody [EPR17880] (ab202420) at 1/2000 dilutionLane 1 : C6 (Rat glial tumor cells) cell lysateLane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells, untreated or treated with etoposide (30ug/ml) for 8 hours, labeling Caspase-2 with ab202420 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on Jurkat cell line. The expression decreased after treatment with etoposide (30ug/ml) for 8 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab202420 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat
Caspase-2 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab202420 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab202420 at 1/2000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HEK-293 whole cell lysate 10 µg (Input). Lane 2: ab202420 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202420 in HEK-293 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.