Anti-Caspase-2 antibody [EPR17881]

• Supplier: Abcam
• Catalog: ab182738
• Prices: $382.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17881
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant fragment within Human Caspase-2 aa 150-350
• Description: Rabbit Monoclonal
• Gene: CASP2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilutionLane 1 : Jurkat whole cell lysates(Human T cell leukemia cells from peripheral blood) treated with 25uM etoposide for 5 hoursLane 2 : Untreated Jurkat whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilutionLane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysatesLane 2 : SK-BR-3 (Human mammary gland adenocarcinoma) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/1000 dilution + Human thymus at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-2 with ab182738 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Jurkat cells is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows;1. ab182738 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.