Anti-Caspr2 antibody
| Name | Anti-Caspr2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33994 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F WB IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Mouse Caspr2 |
| Description | Rabbit Polyclonal |
| Gene | CNTNAP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Caspr2 antibody (ab33994) at 1 µg/mlLane 1 : Brain (Mouse) Tissue Lysate at 20 µgLane 2 : Brain (Rat) Tissue Lysate at 20 µgLane 3 : Brain (Human) Tissue Lysate at 20 µgLane 4 : Sciatic Nerve (Mouse) Lysate at 7.5 µgLane 5 : Sciatic Nerve (Rat) Lysate at 20 µgSecondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
![Caspr2 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Caspr2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33994.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 152kDa: Caspr2.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21431_Caspr2-Primary-antibodies-ab33994-4.jpg)
Caspr2 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Caspr2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33994.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 152kDa: Caspr2.
ICC/IF image of ab33994 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33994, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
Membrane domain organization of myelinated axons requires betaII spectrin. - Membrane domain organization of myelinated axons requires betaII spectrin.
Zhang C, Susuki K, Zollinger DR, Dupree JL, Rasband MN. J Cell Biol. 2013 Nov 11;203(3):437-43.
Investigation of LGI1 as the antigen in limbic encephalitis previously attributed - Investigation of LGI1 as the antigen in limbic encephalitis previously attributed
Lai M, Huijbers MG, Lancaster E, Graus F, Bataller L, Balice-Gordon R, Cowell JK, Dalmau J. Lancet Neurol. 2010 Aug;9(8):776-85.
Genome-wide association study with DNA pooling identifies variants at CNTNAP2 - Genome-wide association study with DNA pooling identifies variants at CNTNAP2
Krumbiegel M, Pasutto F, Schlotzer-Schrehardt U, Uebe S, Zenkel M, Mardin CY, Weisschuh N, Paoli D, Gramer E, Becker C, Ekici AB, Weber BH, Nurnberg P, Kruse FE, Reis A. Eur J Hum Genet. 2011 Feb;19(2):186-93.