Anti-Caspr2 antibody [EPR8738] (ab137052) at 1/1000 dilution (purified) + Human glioma tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-Caspr2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-Caspr2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-Caspr2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab137052 at a working dilution of 1 in 2000. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
All lanes : Anti-Caspr2 antibody [EPR8738] (ab137052) at 1/1000 dilution (unpurified)Lane 1 : Fetal brain lysateLane 2 : Human glioma lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Caspr2 with unpurified ab137052 at dilution 1/250.