Anti-Cathepsin K antibody
| Name | Anti-Cathepsin K antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab19027 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Rat, Human, Zebrafish |
| Antigen | Synthetic peptide, corresponding to a region within amino acids 300-329 of Rat Cathepsin K which includes residue 321 |
| Blocking Peptide | Cathepsin K peptide |
| Description | Rabbit Polyclonal |
| Gene | CTSK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab19027 staining Cathepsin K in mouse bone tissue section, in which human MDA-MB-231 Breast Cancer cells were grown, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation and then blocking with 10% Casein for 30 minutes at 22°C was performed. The primary antibody was diluted 1/200 and incubated with sample in 10% Casein for 17 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.See Abreview
ab19027 staining Cathepsin K in rat osteoclasts by Immunohistochemistry (Frozen sections). 21 day old rat bone was fixed in methanol and then blocked using 20% serum for 30 minutes at 22°C. Samples were then incubated with primary antibody at 1/50 for 3 hours at 22°C. The secondary antibody used was an anti-rabbit IgG conjugated to Cy3® (red) used at a 1/1000 dilution.
ab19027 staining Cathepsin K in cultured human osteoclasts by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100 PBS, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab19027 at 2µg/ml for 30 minutes at 25°C. The secondary used was a FITC conjugated goat anti-rabbit polyclonal used at a 1/00 dilution.Red = ActinBlue = DAPIGreen / Yellow = Cathepsin KSee Abreview
ab19027 staining Cathepsin K in the Human cell line HepaRG by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.1% and blocked with 1% milk for 30 minutes at 21°C. Samples were incubated with primary antibody (1ug/ml in 1% milk) for 30 minutes. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal(1/400) was used as the secondary antibody.See Abreview
Product References
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Genetic and intervention studies implicating complement C3 as a major target for - Genetic and intervention studies implicating complement C3 as a major target for
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Ultraviolet A-induced cathepsin K expression is mediated via MAPK/AP-1 pathway in - Ultraviolet A-induced cathepsin K expression is mediated via MAPK/AP-1 pathway in
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Effects of omega3- and omega6-polyunsaturated fatty acids on RANKL-induced - Effects of omega3- and omega6-polyunsaturated fatty acids on RANKL-induced
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The actin binding protein adseverin regulates osteoclastogenesis. - The actin binding protein adseverin regulates osteoclastogenesis.
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Immune complex-induced inhibition of osteoclastogenesis is mediated via - Immune complex-induced inhibition of osteoclastogenesis is mediated via
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A soft coral-derived compound, 11-epi-sinulariolide acetate suppresses - A soft coral-derived compound, 11-epi-sinulariolide acetate suppresses
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Excessive activity of cathepsin K is associated with cartilage defects in a - Excessive activity of cathepsin K is associated with cartilage defects in a
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