All lanes : Anti-Cathepsin L antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified)Lane 1 : A549 cell lysateLane 2 : HCT-116 cell lysateLane 3 : NIH/3T3 cell lysateLane 4 : PC-12 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Anti-Cathepsin L antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified) + HepG2 cell lysate at 10 µgSecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with unpurified ab133641 at a 1/50 dilution.
Unpurified ab133641 staining Cathepsin L in Human HepaRG cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 30 minutes. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD