All lanes : Anti-Cathepsin W [EPR15135] antibody (ab191083) at 1/50000 dilutionLane 1 : HuT 78 cell lysateLane 2 : MOLT4 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab191083 at 1/30 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-Cathepsin W [EPR15135] antibody (ab191083) at 1/10000 dilution + PC12 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-Cathepsin W [EPR15135] antibody (ab191083) at 1/20000 dilutionLane 1 : A431 cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution