ab85491 staining CaV1.3 in mouse backskin tissue by IHC-P (Bouin's fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval using microwave in citrate buffer. The primary antibody was used at 1/100 dilution and then sections were incubated with Fluorophore conjugated goat anti mouse at 1/50 dilution.
ab85491 staining CaV1.3 in human differentiated iPS cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Tween-20 and then blocked using 3% BSA for 1 hour at 21°C. Samples were then incubated with primary antibody at a 1/100 dilution for 12 hours at 4°C. The secondary antibody used was a anti-mouse IgG (H+L) conjugated to Alexa Fluor® 594 (pink) used at a 1/400 dilution.See Abreview
![Overlay histogram showing SH-SY5Y cells stained with ab85491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85491, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21757_CaV13-Primary-antibodies-ab85491-6.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab85491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85491, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.