Anti-Caveolin-1 antibody [7C8]

• Supplier: Abcam
• Catalog: ab17052
• Prices: $398.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 7C8
• Applications: ICC/IF ICC/IF FC WB IP
• Species Reactivities: Mouse, Rat, Hamster, Human, Pig
• Antigen: Tissue, cells or virus corresponding to Rat Caveolin-1
• Description: Mouse Monoclonal
• Gene: CAV1
• Conjugate: Unconjugated

Product images

ab17052 at a 1/500 dilution staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody. Bound antibody was detected using a FITC conjugated goat anti-mouse antibody.See Abreview

 ab17052 staining Caveolin-1 in human Hacat keratinocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/75 for 24 hours at 4°C. The secondary antibody used was conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.See Abreview

Overlay histogram showing CHO cells stained with ab17052 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17052, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in CHO cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Product References

Peroxynitrite disrupts endothelial caveolae leading to eNOS uncoupling and - Peroxynitrite disrupts endothelial caveolae leading to eNOS uncoupling and

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IGF-IR internalizes with Caveolin-1 and PTRF/Cavin in HaCat cells. - IGF-IR internalizes with Caveolin-1 and PTRF/Cavin in HaCat cells.

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