Anti-CBR1 antibody
| Name | Anti-CBR1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4148 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: HGQFVSEKRVEQW, corresponding to C terminal amino acids 265-277 of Human CBR1 |
| Description | Goat Polyclonal |
| Gene | CBR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab4148 (3ug/ml) staining human CBR1 in human kidney by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
ICC/IF image of ab4148 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4148, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lanes 1 & 10 : Protein MarkerLanes 2 - 9 : Anti-CBR1 antibody (ab4148) at 1/1000 dilution (in PBS + (0.05%) Tween at 25°C )Lane 1 : Protein MarkerLane 2 : Whole cell lysate of human lung cancer cell line A549 at 25 µgLane 3 : Whole cell lysate of human lung cancer cell line A549 at 25 µgLane 4 : Whole cell lysate of human lung cancer cell line A549 at 25 µgLane 5 : Whole cell lysate of human lung cancer cell line A549 at 25 µgLane 6 : 1X SDS Loading BufferLane 7 : recombinant CBR1 at 0.03 µgLane 8 : recombinant CBR1 at 0.07 µgLane 9 : recombinant CBR1 at 0.1 µgLane 10 : Protein MarkerSecondaryLanes 2 - 9 : An HRP-conjugated anti-goat IgG at 1/10000 dilutiondeveloped using the ECL technique
Product References
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