ab92576, at 1/50 dilution, staining CBR1 in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry (Foramlin/PFA-fixed paraffin-embedded sections). Lefthand image without peptide and righthand image with peptide.
All lanes : Anti-CBR1 antibody (ab92576) at 1/500 dilutionLane 1 : HeLa cellsLane 2 : HeLa cells with Peptide at 10 µgLysates/proteins at 30 µg per lane.
ICC/IF image of ab92576 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92576, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.