Anti-CBR1 antibody [4E12]
| Name | Anti-CBR1 antibody [4E12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab111347 |
| Prices | $378.00 |
| Sizes | 50 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 4E12 |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-277 of Human CBR1, purified from E |
| Description | Mouse Monoclonal |
| Gene | CBR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab111347 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111347, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-CBR1 antibody [4E12] (ab111347) at 1/1000 dilutionLane 1 : HeLa Cell LysateLane 2 : HepG2 Cell LysateLane 3 : 293T Cell LysateLysates/proteins at 40 µg per lane.developed using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21992_CBR1-Primary-antibodies-ab111347-3.jpg)
All lanes : Anti-CBR1 antibody [4E12] (ab111347) at 1/1000 dilutionLane 1 : HeLa Cell LysateLane 2 : HepG2 Cell LysateLane 3 : 293T Cell LysateLysates/proteins at 40 µg per lane.developed using the ECL technique