Anti-CBS antibody
| Name | Anti-CBS antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab96252 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human, Zebrafish, Mouse, Rat, Rabbit, Bovine |
| Antigen | Recombinant fragment corresponding to a region within amino acids 39-221 of Human CBS (AAH00440) |
| Description | Rabbit Polyclonal |
| Gene | CBS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilutionLane 1 : Molt-4 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft using ab96252 at 1/500 dilution.
ICC/IF image of ab96252 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96252, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-CBS antibody (ab96252) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate at 20 µgLane 3 : Zebrafish heart homogenate at 20 µgLane 4 : Zebrafish liver homogenate at 20 µgLane 5 : Human epithelial carcinoma cell line at 20 µgSecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.