Anti-CBS antibody (ab135626) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg
All lanes : Anti-CBS antibody (ab135626) at 1/100 dilutionLane 1 : nontransfected 293 cells lysateLane 2 : lysate of 293 cells transiently transfected with the CBS gene Lysates/proteins at 2 µg per lane.
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labelling CBS with ab135626 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of ab135626 staining CBS at 1/10 in 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CBS using ab135626 at 1/10 dilution in 293 cell followed by Alexa Fluor 488 conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).