Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade
| Name | Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab10811 |
| Prices | $385.00 |
| Sizes | 1 ml |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | MAC353 |
| Applications | IHC-F ELISA WB ICC/IF ICC/IF ChIP IP |
| Species Reactivities | Mouse, Human |
| Antigen | 71 amino acid fusion protein corresponding to the c-terminal region of M31 (human/mouse) |
| Description | Rat Monoclonal |
| Gene | CBX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
HeLA cells were stained with ab10811 in panel one. In panel two they were stained with ab10811 (green), DAPI (blue) and SH-CREST (red), which stains the centromeres.Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeablize 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4oC diluted 1/100 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen. Notes: Ample washing between each step. TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2.
Immunofluorescence analysis of mouse erythroleukemia cell nuclei, staining CBX1 / HP1 beta in heterochromatin, with ab10811. Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and blocked for 30 min with 2.5% BSA. Cells were incubated with primary antibody (1/50) before incubating with a Cy3-conjugated goat anti-rat IgG to detect staining.
Product References
Dynamics of the two heterochromatin types during imprinted X chromosome - Dynamics of the two heterochromatin types during imprinted X chromosome
Vaskova EA, Dementyeva EV, Shevchenko AI, Pavlova SV, Grigor'eva EV, Zhelezova AI, Vandeberg JL, Zakian SM. PLoS One. 2014 Feb 4;9(2):e88256.
Heterochromatin protein 1 expression is reduced in human thyroid malignancy. - Heterochromatin protein 1 expression is reduced in human thyroid malignancy.
Tretiakova MS, Bond SD, Wheeler D, Contreras A, Kocherginsky M, Kroll TG, Hale TK. Lab Invest. 2014 Jul;94(7):788-95.
SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction - SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction
Mund A, Schubert T, Staege H, Kinkley S, Reumann K, Kriegs M, Fritsch L, Battisti V, Ait-Si-Ali S, Hoffbeck AS, Soutoglou E, Will H. Nucleic Acids Res. 2012 Dec;40(22):11363-79.
Histone H4-lysine 20 monomethylation is increased in promoter and coding regions - Histone H4-lysine 20 monomethylation is increased in promoter and coding regions
Talasz H, Lindner HH, Sarg B, Helliger W. J Biol Chem. 2005 Nov 18;280(46):38814-22. Epub 2005 Sep 15.
Histone H4 hyperacetylation precludes histone H4 lysine 20 trimethylation. - Histone H4 hyperacetylation precludes histone H4 lysine 20 trimethylation.
Sarg B, Helliger W, Talasz H, Koutzamani E, Lindner HH. J Biol Chem. 2004 Dec 17;279(51):53458-64. Epub 2004 Sep 28.