All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/mlLane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)Lane 5 : MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196)Lane 6 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![cbx2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to cbx2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab80044.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 52kDa: cbx2.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/22101_CBX2-Primary-antibodies-ab80044-7.jpg)
cbx2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to cbx2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab80044.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 52kDa: cbx2.