ab62074 staining CCK4 in E9.5 mouse embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 5% donkey serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.Image shows sagittal section of E9.5 mouse embryo. Neuroectoderm and underlying mesoderm labelled with ab62074 in green. DAPI stained nuclei in blue.See Abreview
All lanes : Anti-CCK4 antibody (ab62074) at 1 µg/mlLane 1 : Human colon tissue lysateLane 2 : Mouse colon tissue lysateLane 3 : Rat colon tissue lysateLysates/proteins at 10 µg per lane.Secondaryanti-rabbit IgG
ab62074 at 5 µg/ml staining CCK4 in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).