ab117412 staining CCK4 (green) in wild type mouse embryonic stem cells (D3) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with 0.1% Triton X-100 and blocked with 1% serum for 30 minutes at 16°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 2 hour at 16°C. ab15007, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.See Abreview