All lanes : Anti-CCK4 antibody - N-terminal (ab135675) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysate Lane 2 : 293 cell lysate transiently transfected with the CCK4 geneLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue labelling CCK4 with ab135675 at 1/50 dilution, followed by peroxidase-conjugated to the secondary antibody, and DAB staining.
Flow cytometric analysis of NCI-H460 cells labelling CCK4 with ab135675 at 1/10 dilution (green) compared to a negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.