Anti-CCKAR antibody
| Name | Anti-CCKAR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab77269 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide: C-FEASQKKSAKERKPST , corresponding to internal sequence amino acids 242-257 of Human CCKAR (NP_000721 |
| Description | Goat Polyclonal |
| Gene | CCKAR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab77269 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77269 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab77269 at 10µg/ml staining CCKAR in human pancreas by Immunohistochemistry, Paraffin-embedded tissue (note cytoplasmic staining of some cells in the islets of Langerhans).